Polymerization of Gag on the inner leaflet of the plasma membrane drives the assembly of Human Immunodeficiency Virus 1 (HIV-1).Gag recruits components of the endosomal sorting complexes required for transport (ESCRT) to facilitate membrane fission and virion release.ESCRT assembly is initiated by recruitment of ALIX and TSG101/ESCRT-I, which Whey Protein bind directly to the viral Gag protein and then recruit the downstream ESCRT-III and VPS4 factors to complete the budding process.In contrast to previous models, we show that ALIX is recruited transiently at the end of Gag assembly, and that most ALIX Leg Warmers molecules are recycled into the cytosol as the virus buds, although a subset remains within the virion.Our experiments imply that ALIX is recruited to the neck of the assembling virion and is mostly recycled after virion release.